Keywords: Gold Nanoprism; Green Synthesis; Penicillium citrinum; Transmission [15], triangular gold nano plates using algal solutions of. [14], S. S. Shankar, A. Rai, B. Ankamwar, A. Singh, A. Ahmad and M. Sastry, “ Biological Synthesis of Triangular Gold Nanoprisms,” Nature Materials, Vol. 3, No . Synthesis of triangular gold nanoprism using lemon grass leaf extract showed UV-Visible spectra of gold nanoparticles by bio-reduction of Chloroauric acid in.

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However, bio application of large nano particles greater than 50 nm are limited due to reduced endocytosis and poor renal clearance [30].

The GNPs then resuspended in double distilled water and again centrifuged. There are other reports which showed the intracellular synthesis of spherical GNPs in live Alfaalfa plants [12], truncated triangular and spherical GNPs from sun dried Cinamonum camphora leaf extract [13], triangular gold nanoprism from lemon grass extract [14], hexagonal, prism and spherical shaped GNPs using plant surfactants [15], triangular gold fold plates using algal solutions of Chlorella vulgaris [16] and Sargassum sp.

AFM was conducted to record the morphological changes of fungal mycelia following GNP synthesis on their surface. A Formation of GNPs on mycelial biomass resulting purple color. We emphasized on bio-inspired synthesis of gold nanoparticles by Penicillium citrinum MTCC in room temperature.

FTIR analysis also showed that protein molecules were involved in stabilization. The chemical synthesis of metal nanoprisms ov large quantities using sphere-triangle shape transformation [] or seeded growth [29] has been found to be more difficult. However, there is limited information available regarding the synthesis of GNPs using fungus.

Sample images were processed using ProScan image processing software. Metal nanoparticles are produced mainly by means of chemical processes involving chemical aggressive nnoprisms agents e.

Thus d-spacing of plane of gold nanoprism revealed a deviation from the standard value of fcc gold crystal 2. The fringe spacing was 2.

Gold nanoparticles GNPs synthesized on cell surface were isolated by placing the mycelial suspension on a bath sonicator and sonicated for 15 minutes.

Figure 1 B represents GNPs solution collected from mycelial surfaces following sequential sonication and filtration. A higher magnification image of gold nanoprism is shown in Figure C. B A selected syntbesis electron diffraction pattern of GNPs. The ring structure of SAED pattern is a characteristic of polycrystalline gold Recently cetyltrimethylammonium bromide CTAB has been shown to induce the formation of gold nanorods, triangular prism and spheres through seed mediated approaches depending on iodide contamination present in CTAB which act as shape directing agent [35].


Thus a visual inspection was routinely performed. Formation of gold nanoparticles resulted in a sharp colour change from yellow to dark pinkish. This report provides for the first time rapid novel environment friendly cell surface based synthesis of stable gold nanoprisms at room temperature nanpprisms Penicillium citrinum MTCC biomass.

For FTIR spectrum analysis the GNPs were centrifuged at 10, rpm for 10 minutes to remove free proteins or other compounds present in the solution. The physical nature of GNPs indicated that most of the nanoparticles were prism shaped. This microbial synthesis of GNPs could be explained by the fact that microorganisms often face metal ions or metals in their environment and thereby developed mechanism to detoxify metals by changing the redox states of metal ions and or syntgesis precipitation of metals forming a basis for synthesis of nanoparticles [32].

B Color of GNPs solution. To investigate the bio,ogical nature of gold nanoparticles transmission electron microscopic syntehsis was done.

Moreover, the synthesis of other anisotropic biologgical of gold like nano rod, cube, triangle which are present in smaller quantities as per TEM images may be achieved by further tuning the synthesis protocol using the fungus.

It was quite exciting for us to find this bioprocess where mycelial biomass suspended in double distilled water in presence of chloroauric acid could produce GNPs on their surfaces. The selected area electron diffraction pattern SAED of gold nanoprism Figure 4 B indicated that the entire nanoparticle was nanocrystalline in nature [31]. The Scherrer ring patterns indicate the fcc gold which is nanocrystalline in nature.

Synthesis of triangular gold nanoprism using lemon grass leaf extract showed large size of nanoprisms ranging from – nm length and truncated vertices [14].

Biological Synthesis of Colloidal Gold Nanoprisms Using Penicillium citrinum MTCC

Biological systems are capable of synthesizing a remarkably diverse range of inorganic materials. In recent years the synthesis of stable gold nanoparticles is biilogical of the most active areas of research in the field of nanotechnology because of their applications in a number of areas like bio-imaging, bio-medicine, bio-sensors, bio-labels, catalysis and others [].

The zeta potential of GNPs was measured to know their colloidal stability and the nature of the charge carried in their surface. Each experiment was repeated thrice using freshly grown culture of P.

In all these respects biological approach would become a better choice for the synthesis of gold nanoparticles GNPs. Received January 16 th; revised February 23 rd; accepted April triangula th FTIR spectroscopy data also supported the fact that biomolecules were present in the surface. FTIR spectra of hriangular nanoparticles. The surface plasma resonance of GNPs at nm was due to collective coherent electronic oscillation of metal nanopartticles in presence of electromagnetic field of light.


Therefore a growing demand has been accumulated to develop environment friendly approaches to synthesize nanomaterials Green Chemistry.

Biological synthesis of triangular gold nanoprisms.

Development of strain in crystals of gold nanoprism was observed from the analysis of crystals. All chemical reagents were purchased from Sigma St. The synthesis of nanoparticles using biological approaches offers several advantages than chemical synthesis like environmentally acceptable solvent, eco friendly reducing and capping agents.

The arrow in F indicates the splitting of spots in the FFT image of gold nanoprism. Typically 2 g biomass biologiccal weight was dispersed in 20 ml of double distilled water pH 5.

Biomaterials have inspired nanporisms growing research effort in which biomolecules are used to synthesize and assemble materials in the laboratory. The process trixngular repeated for three biplogical and finally the centrifuged part containing GNPs were redispersed in double distilled water and subjected to FTIR spectroscopy.

FFT ssynthesis also showed an elongation of the diffraction reflexes. GNPs size was found to be in the range of 10 – 40 nm with maximum population centered on 20 nm Figure 2. The UV-Visible spectral scan of dispersed gold nanoparticles GNPs solution showed absorption maxima at nm due to surface plasma resonance SPR of gold nanoparticles.

The selected area electron diffraction pattern SAED of gold nanoprism Figure 4 B indicated that the entire nanoparticle was nanocrystalline in nature So it can be assumed that a micro stress was developed due to the formation of anisotropic nanoprism.

Due to symmetric face centered nanoprsims fcc lattice of gold nanoparticles, the formation of anisotropic structures like cube, prism, star, branched nanoparticles require a selective capping agent during growth In addition gold nanoprism offers several advantages in biomedical applications.

Synthesis of spherical gold nanoparticles GNPs have been reported using the fungus Fusarium oxysporum and Verticillium sp.